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bioRxiv · 10.1101/2022.10.27.514107

Iterative remodeling of the mouse uterus requires Hedgehog signaling

Abstract

The adult uterus regenerates in the human during the menstrual cycle, and remodels in the mouse during the estrous cycle. Decades of work has demonstrated that this process is controlled by cycling steroid hormones, estrogen and progesterone. However, downstream signaling pathways that link hormonal action to this regeneration and remodeling are yet to be identified in the cycling uterus. We set out to identify these pathways, with the overarching hypothesis that developmental signaling pathways are redeployed in the adult uterus to control remodeling in the mouse. We were surprised to find that the majority of Hedgehog signaling components were transcriptionally co-regulated throughout the estrous cycle. To test the role of Hh signaling in cyclical uterine remodeling, we conditionally knocked out the major activator of the pathway, smoothened (Smo) using the progesterone receptor cre (PR-Cre). In the absence of Hh signaling, the uterus no longer remodels throughout the estrous cycle. We also show that the smooth muscle fibers of the uterus are significantly larger in the conditional knockouts compared to the controls suggesting hypertrophy of the smooth muscle. Our findings support the possibility that this smooth muscle homeostasis may underlie important aspects of uterine function such as contractility during late-stage pregnancy or the development of uterine smooth muscle tumors.

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Roberson, E. C., Tran, N. K., Godambe, A., Rust, T., Nguimtsop, M., Mark, H., Fitch, R., Wallingford, J. B.. 2022-10-29. Iterative remodeling of the mouse uterus requires Hedgehog signaling. https://doi.org/10.1101/2022.10.27.514107

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