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bioRxiv · 10.1101/2022.10.26.513827

IntestLine: a Shiny-based application to map the rolled intestinal tissue onto a line

Yuzeir, A.·Bejaran, D.·Grein, S.·Hasenauer, J.·Schlitzer, A.·Yu, J.
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Abstract

To allow the comprehensive histological analysis of the whole intestine in one image, the tissue is often rolled to a spiral before imaging. This Swiss-rolling technique facilitates robust experimental procedures, but it limits the possibilities to comprehend changes along the intestine. Here, we present IntestLine, a Shiny-based open-source application to map imaging data of intestinal tissues in spiral shape onto a line. The mapping of intestinal tissues improves the visualization of the whole intestine in both proximal-distal and serosa-luminal axis, and facilitates the observation of location-specific cell types and markers. In summary, IntestLine serves as a tool to visualize and characterize intestine in future imaging studies.

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Yuzeir, A., Bejaran, D., Grein, S., Hasenauer, J., Schlitzer, A., Yu, J.. 2022-10-27. IntestLine: a Shiny-based application to map the rolled intestinal tissue onto a line. https://doi.org/10.1101/2022.10.26.513827

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