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bioRxiv · 10.1101/2022.10.20.513000

T-RHEX-RNAseq - A tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells.

Gustafsson, C.·Hauenstein, J.·Frengen, N.·Krstic, A.·Luc, S.·Mansson, R.
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Abstract

BackgroundRNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming - to allow analysis of a broader range of transcripts - remains challenging. ResultsWe here describe a tagmentation-based, rRNA blocked, random hexamer primed RNAseq approach (T-RHEX-RNAseq) for generating stranded RNAseq libraries from very low numbers of FACS sorted cells without RNA purification steps. ConclusionT-RHEX-RNAseq provides an easy-to-use, time efficient and automation compatible method for generating stranded RNAseq libraries from rare cells.

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Gustafsson, C., Hauenstein, J., Frengen, N., Krstic, A., Luc, S., Mansson, R.. 2022-10-21. T-RHEX-RNAseq - A tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells.. https://doi.org/10.1101/2022.10.20.513000

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