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bioRxiv · 10.1101/2022.10.19.512892

High performance genetically-encoded green fluorescent biosensors for intracellular L-lactate

Le, G. N. T.·Hario, S.·Takahashi-Yamashiro, K.·Li, S.·Drobizhev, M.·Nasu, Y.·Campbell, R. E.
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Abstract

L-Lactate is a monocarboxylate produced during the process of cellular glycolysis and has long been generally considered a waste product. However, studies in recent decades have provided new perspectives on the physiological roles of L-lactate as a major energy substrate and a signaling molecule. To enable further investigations of the physiological roles of L-lactate, we have developed a series of high-performance ({Delta}F/F = 15 to 30 in vitro), intensiometric, genetically-encoded green fluorescent protein (GFP)-based intracellular L-lactate biosensors with a range of affinities. We evaluated the performance of these biosensors by in vitro and live-cell characterization and demonstrated the utility with imaging applications in several cell lines.

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Le, G. N. T., Hario, S., Takahashi-Yamashiro, K., Li, S., Drobizhev, M., Nasu, Y., Campbell, R. E.. 2022-10-20. High performance genetically-encoded green fluorescent biosensors for intracellular L-lactate. https://doi.org/10.1101/2022.10.19.512892

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