Ploidy modulates cell size and metabolic rate in Xenopus embryos

Abstract

A positive correlation between genome size and cell size is well documented, but impacts on animal physiology are poorly understood. In Xenopus frogs, the number of genome copies (ploidy) varies across species and can be manipulated within a species. Here we show that triploid tadpoles contain fewer, larger cells than diploids and consume oxygen at a lower rate. Treatments that altered cell membrane stability or electrical potential abolished this difference, suggesting that a decrease in total cell surface area reduces basal energy consumption in triploids. Comparison of Xenopus species that evolved through polyploidization revealed that metabolic differences emerged during development when cell size scaled with genome size. Thus, ploidy affects metabolism by altering the cell surface area to volume ratio in a multicellular organism. One-Sentence SummaryThe amount of DNA per cell in a vertebrate modulates basal metabolism by altering cell size and plasma membrane energetics.