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bioRxiv · 10.1101/2022.10.17.512483

Timing of TORC1 inhibition dictates Pol III involvement in longevity in Caenorhabditis elegans

Malik, Y.·Silva, I. G.·Diazgranados, R. R.·Selman, C.·Alic, N.·Tullet, J. M. A.
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Abstract

Organismal growth and lifespan are inextricably linked. Target of Rapamycin (TOR) signalling regulates protein production for growth and development, but if reduced extends lifespan across species. Reduction of the enzyme RNA polymerase III, which transcribes tRNAs and 5S rRNA, also extends longevity. Here, we identify a temporal genetic relationship between TOR and Pol III in C. elegans, showing that they collaborate to regulate progeny production and lifespan. Interestingly, the lifespan interaction between Pol III and TOR is only revealed when TOR signaling is reduced specifically in adulthood demonstrating the importance of timing to control TOR regulated developmental vs adult programs. Additionally, we show that Pol III acts in C. elegans muscle to promote both longevity and healthspan and that reducing Pol III even in late adulthood is sufficient to extend lifespan. This demonstrates the importance of Pol III for lifespan and age-related health in adult C. elegans.

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Malik, Y., Silva, I. G., Diazgranados, R. R., Selman, C., Alic, N., Tullet, J. M. A.. 2022-10-17. Timing of TORC1 inhibition dictates Pol III involvement in longevity in Caenorhabditis elegans. https://doi.org/10.1101/2022.10.17.512483

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