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bioRxiv · 10.1101/2022.10.06.511114

Live Cell Multicolour Lifetime Imaging Using Genetically Encodable Fluorophores

Starling, T.·Carlon-Andres, I.·Iliopoulou, M.·Williamson, D. J.·Padilla-Parra, S.
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Abstract

Nine fluorescent proteins (FPs) simultaneous imaging has been demonstrated in a single acquisition using fluorescence lifetime imaging microscopy (FLIM) combined with pulsed interleaved excitation (PIE) for three laser lines. We also show how to unmix spectrally similar FPs in a pixel-by-pixel manner with an analytical non-fitting solution.

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Starling, T., Carlon-Andres, I., Iliopoulou, M., Williamson, D. J., Padilla-Parra, S.. 2022-10-07. Live Cell Multicolour Lifetime Imaging Using Genetically Encodable Fluorophores. https://doi.org/10.1101/2022.10.06.511114

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