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bioRxiv · 10.1101/2022.10.06.511111

Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST - RE1-Silencing Transcription factor

Veverka, P.·Brom, T.·Janovic, T.·Stojaspal, M.·Pinkas, M.·Novacek, J.·Hofr, C.
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Abstract

The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and diseases.

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Veverka, P., Brom, T., Janovic, T., Stojaspal, M., Pinkas, M., Novacek, J., Hofr, C.. 2022-10-06. Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST - RE1-Silencing Transcription factor. https://doi.org/10.1101/2022.10.06.511111

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