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bioRxiv · 10.1101/2022.10.04.510822

Nuclear autophagy interactome unveils WSTF as a constitutive nuclear inhibitor of inflammation

Abstract

Macroautophagy (hereafter referred to as autophagy) degrades a variety of cellular components. A poorly understood area is autophagic degradation of nuclear substrates, or "nuclear autophagy". It remains unclear what can be degraded by autophagy from the mammalian nuclei. We began our study by investigating the nuclear binding partners of ATG8 family proteins that play important roles in recognizing autophagy substrates. We systematically evaluated the ATG8 nuclear interactome in primary human cells and in mouse brain, identifying hundreds of novel interactions. We continued our study by evaluating the nuclear proteomes of cellular senescence, a stable form of cell cycle arrest program associated with inflammation, in which nuclear autophagy is involved. Combined with the ATG8 nuclear interactome data, we identified WSTF, a component of the ISWI chromatin remodeling complex, as a novel substrate of nuclear autophagy. The degradation of WSTF, mediated by a direct interaction with the GABARAP isoform of ATG8, promotes chromatin accessibility of inflammatory genes and induces senescence-associated inflammation. Furthermore, WSTF directly binds the p65 subunit of NF-{kappa}B and inhibits its acetylation, thus blocking inflammatory gene expression in the setting of senescence, cancer, and pathogen infection. In addition, we show that loss of WSTF is required for the immuno-surveillance of oncogenic Ras in mouse liver; forced expression of WSTF inhibited tumor-suppressive inflammation and led to the development of liver tumors. Taken together, our study provides a global view of mammalian nuclear autophagy and reveals a novel nuclear inhibitor of inflammation implicated in diverse pathological contexts. Targeting WSTF may be broadly valuable as therapeutic intervention of inflammatory diseases.

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BibTeXRIS

Wang, Y., Eapen, V. V., Kournoutis, A., Onorati, A., Li, X., Zhou, X., Cetinbas, M., Wang, L., Liu, J., Bretz, C., Zhou, Z., Ho Sui, S. J., Saladi, S. V., Sadreyev, R., Adams, P. D., Kingston, R. E., Yue, Z., Johansen, T., Dou, Z.. 2022-10-05. Nuclear autophagy interactome unveils WSTF as a constitutive nuclear inhibitor of inflammation. https://doi.org/10.1101/2022.10.04.510822

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