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bioRxiv · 10.1101/2022.10.04.510646

α-synuclein fibril and synaptic vesicle interactions lead to vesicle destruction and increased uptake into neurons

Abstract

Monomeric alpha-synuclein (aSyn) is a well characterised as a lipid binding protein. aSyn is known to form amyloid fibrils which are also localised with lipids and organelles in so called Lewy bodies, insoluble structures found in Parkinsons disease patients brains. It is still unclear under which conditions the aSyn-lipid interaction can start to become pathological. Previous work to address pathological interactions has focused on using synthetic lipid membranes, which lack the complexity of physiological lipid membranes which not only have a more complex lipid composition, but also contain lipid interacting proteins. Here, we investigate how either monomeric or fibrillar aSyn interact with physiological synaptic vesicles (SV) isolated from rodent brain. Using small angle neutron scattering and high-resolution imaging we observe that aSyn fibrils disintegrate SV, whereas aSyn monomers cause clustering of SV. Furthermore, SV enhance the aggregation rate of aSyn, however increasing the SV:aSyn ratio causes a reduction in aggregation propensity. SV lipids appear as an integrated part of aSyn fibrils and while the fibril morphology differs to aSyn fibrils alone, the core fibril structure remains the same. We finally demonstrate that lipid-associated aSyn fibrils are more easily taken up into cortical i3Neurons derived from induced pluripotent stem cells. Our study sheds light on differences between interactions of aSyn with synthetic lipid vesicles and physiological SV. We show how aSyn fibrils may enhance pathology by disintegrating SV, which in turn may have fatal consequences for neurons. Furthermore, disease burden may additionally be impacted by an increased uptake of lipid-associated aSyn by neurons, leading to more SV damage and enhancing aSyn aggregation.

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BibTeXRIS

Stephens, A. D., Fernandez-Villegas, A., Chung, C. W., Vanderpoorten, O., Pinotsi, D., Mela, I., Ward, E. N., McCoy, T. M., Cubitt, R., Routh, A. F., Kaminski, C. F., Schierle, G. S. K.. 2022-10-06. α-synuclein fibril and synaptic vesicle interactions lead to vesicle destruction and increased uptake into neurons. https://doi.org/10.1101/2022.10.04.510646

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