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bioRxiv · 10.1101/2022.09.28.509965

ClickArr: a novel, high throughput assay for evaluating β-arrestin isoform recruitment

French, A. R.·Meqbil, Y. J.·van Rijn, R. M.
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Abstract

Modern methods for quantifying signaling bias at GPCRs rely on using a single {beta}-arrestin isoform. However, it is increasingly appreciated that the two {beta}-arrestin isoforms have unique roles, requiring the ability to assess {beta}-arrestin isoform preference. Herein, we present ClickArr, a live-cell assay that simultaneously reports recruitment of both {beta}-arrestin isoforms as they compete for interaction with GPCRs. We demonstrate that an agonist can have {beta}-arrestin isoform bias, potentially opening up a new dimension for drug development.

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French, A. R., Meqbil, Y. J., van Rijn, R. M.. 2022-09-30. ClickArr: a novel, high throughput assay for evaluating β-arrestin isoform recruitment. https://doi.org/10.1101/2022.09.28.509965

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