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bioRxiv · 10.1101/2022.09.26.509603

Integrated Model of the Vertebrate Augmin Complex

Travis, S. M.·Mahon, B. P.·Huang, W.·Ma, M.·Rale, M. J.·Kraus, J. S.·Taylor, D.·Zhang, R.·Petry, S.
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Abstract

Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which exponentially amplifies microtubules during cell division. Branching microtubule nucleation relies on the hetero-octameric augmin complex, but understanding how augmin promotes branching has been hindered by a lack of structural information about the complex. Here, we report an integrated model of vertebrate augmin, combining cryo-electron microscopy, advanced protein structural prediction, and the visualization of fused bulky tags via negative stain electron microscopy. This strategy allowed us to identify the location and orientation of each subunit within the structure. Evolutionary analysis of augmins structure reveals that it is highly conserved across diverse eukaryotes, and that augmin contains a previously-unidentified microtubule binding site. Moreover, we identify homology with the kinetochore-localized NDC80 complex. This new model of the augmin complex provides insight towards the mechanism and evolution of branching microtubule nucleation.

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Travis, S. M., Mahon, B. P., Huang, W., Ma, M., Rale, M. J., Kraus, J. S., Taylor, D., Zhang, R., Petry, S.. 2022-09-27. Integrated Model of the Vertebrate Augmin Complex. https://doi.org/10.1101/2022.09.26.509603

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