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bioRxiv · 10.1101/2022.09.26.509532

Clonally related, Notch-differentiated spinal neurons integrate into distinct circuits

Bello-Rojas, S.·Bagnall, M. W.
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Abstract

Shared lineage has diverse effects on patterns of neuronal connectivity. In mammalian cortex, excitatory sister neurons assemble into shared microcircuits, whereas throughout the Drosophila nervous system, Notch-differentiated sister neurons diverge into distinct circuits. Notch-differentiated sister neurons have been observed in vertebrate spinal cord and cerebellum, but whether they integrate into shared or distinct circuits remains unknown. Here we evaluate the connectivity between sister V2a/b neurons in the zebrafish spinal cord. Using an in vivo labeling approach, we identified pairs of sister V2a/b neurons born from individual Vsx1+ progenitors and observed that they have similar axonal trajectories and proximal somata. However, paired whole-cell electrophysiology and optogenetics revealed that sister V2a/b neurons receive input from distinct presynaptic sources, do not communicate with each other, and connect to largely distinct targets. These results resemble the divergent connectivity in Drosophila and represent the first evidence of Notch-differentiated circuit integration in a vertebrate system.

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Bello-Rojas, S., Bagnall, M. W.. 2022-09-27. Clonally related, Notch-differentiated spinal neurons integrate into distinct circuits. https://doi.org/10.1101/2022.09.26.509532

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