Bioefficacy of hydroxytyrosol-rich food supplements on preventing lipid peroxidation in healthy men

Bender, C.; Candi, I.; Rogel, E.

doi:10.1101/2022.09.21.508834

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bioRxiv · 10.1101/2022.09.21.508834

Bioefficacy of hydroxytyrosol-rich food supplements on preventing lipid peroxidation in healthy men

Bender, C.·Candi, I.·Rogel, E.

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Abstract

In the present study we report the efficacy of food matrices derived from olives in preventing oxidation of low-density lipoprotein cholesterol (LDL) and lipid peroxidation. To this end, 12 healthy volunteers were divided into 3 groups and cross-received a single dose of olive phenolics, mainly hydroxytyrosol (HT), provided as a liquid dietary supplement (30.6 or 61.5 mg HT), or as fortified extra virgin olive oil (12.19 mg HT + tyrosol). Blood and urine samples were collected at baseline and up to 12 hours after ingestion. Plasma oxidized LDL levels were measured by ELISA using a monoclonal antibody, while F2-isoprostanes were quantified in urine by UHPLC-MS/MS. Despite the great variability between individuals, a tendency to reduce lipoxidation reactions has been observed after olive phenolics intake in both, blood and urine. In addition, the subgroup of individuals with the highest baseline lipoxidation level showed a decrease in F2-isoprostanes (p < 0.05) after taking the food supplements, as well as a marked decrease in oxidized LDL levels (p < 0.01) after intake of the food supplement with the lowest HT dose. These promising results suggest that HT supplementation could be a useful aid in preventing lipoxidation. Additionally, people with a redox imbalance could benefit even more from supplementing with bioavailable HT.

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Bender, C., Candi, I., Rogel, E.. 2022-09-22. Bioefficacy of hydroxytyrosol-rich food supplements on preventing lipid peroxidation in healthy men. https://doi.org/10.1101/2022.09.21.508834

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