FDA-approved drug screening identified micafungin as an antiviral agent against bat-borne emerging zoonotic Pteropine orthoreovirus

Katta, T.; Sato, A.; Kadofusa, N.; Ishibashi, T.; Shimoda, H.; Iida, A.; Hondo, E.

doi:10.1101/2022.09.21.508823

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bioRxiv · 10.1101/2022.09.21.508823

FDA-approved drug screening identified micafungin as an antiviral agent against bat-borne emerging zoonotic Pteropine orthoreovirus

Katta, T.·Sato, A.·Kadofusa, N.·Ishibashi, T.·Shimoda, H.·Iida, A.·Hondo, E.

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Abstract

Bat-borne emerging zoonotic viruses cause major outbreaks, such as the Ebola virus, Nipah virus, severe acute respiratory syndrome (SARS) coronavirus, and SARS-CoV-2. Pteropine orthoreovirus (PRV), which spillover event occurred from fruit bats to humans, causes respiratory syndrome in humans widely in South East Asia. Repurposing approved drugs against PRV is a critical tool to confront future PRV pandemics. We screened 2,943 compounds in an FDA-approved drug library and identified eight hit compounds that reduce viral cytopathic effects on cultured Vero cells. Real-time quantitative PCR analysis revealed that six of eight hit compounds significantly inhibited PRV replication. Among them, micafungin used clinically as an antifungal drug, displayed a prominent antiviral effect on PRV. HighlightsO_LIA library of 2,943 FDA-approved drugs was screened to find potential antiviral drugs of Pteropine orthoreovirus. C_LIO_LISix hit compounds dramatically inhibited viral replication in vitro. C_LIO_LIMicafungin possessed antiviral activity to multiple strains of PRV. C_LI

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Katta, T., Sato, A., Kadofusa, N., Ishibashi, T., Shimoda, H., Iida, A., Hondo, E.. 2022-09-22. FDA-approved drug screening identified micafungin as an antiviral agent against bat-borne emerging zoonotic Pteropine orthoreovirus. https://doi.org/10.1101/2022.09.21.508823

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