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bioRxiv · 10.1101/2022.09.19.508483

The Mouse N-Glycome Atlas - High-resolution N-glycan analysis of 23 mouse tissues.

Helm, J.·Mereiter, S.·Oliveira, T.·Penninger, J. M.·Altmann, F.·Stadlmann, J.
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Abstract

N-glycosylation is one of the most common protein modifications in eukaryotes, with immense importance at the molecular, cellular, and organismal level. Accurate and reliable N-glycan analysis is essential to obtain a systems-wide understanding of fundamental biological processes. Due to the structural complexity of glycans, their analysis is still highly challenging. Here we make publicly available a consistent N-glycome dataset of 20 different mouse tissues and demonstrate a multimodal data analysis workflow that allows for unprecedented depth and coverage of N-glycome features. This highly scalable, LC-MS/MS data-driven method integrates the automated identification of N-glycan spectra, the application of non-targeted N-glycome profiling strategies and the isomer-sensitive analysis of glycan structures. Our delineation of critical sub-structural determinants and glycan isomers across the mouse N-glycome uncovered tissue-specific glycosylation patterns, the expression of non-canonical N-glycan structures and highlights multiple layers of N-glycome complexity that derive from organ-specific regulations of glycobiological pathways.

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Helm, J., Mereiter, S., Oliveira, T., Penninger, J. M., Altmann, F., Stadlmann, J.. 2022-09-19. The Mouse N-Glycome Atlas - High-resolution N-glycan analysis of 23 mouse tissues.. https://doi.org/10.1101/2022.09.19.508483

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