Multi-Echo Investigations of Positive and Negative CBF and Concomitant BOLD Changes

Abstract

Unlike the positive blood oxygenation level-dependent (BOLD) response (PBR), commonly taken as an indication of an activated brain region, the physiological origin of negative BOLD signal changes (i.e. a negative BOLD response, NBR), also referred to as deactivation is still being debated. In this work, an attempt was made to gain a better understanding of the underlying mechanism by obtaining a comprehensive measure of the contributing cerebral blood flow (CBF) and its relationship to the NBR in the human visual cortex, in comparison to a simultaneously induced PBR in surrounding visual regions. To overcome the low signal-to-noise ratio (SNR) of CBF measurements, a newly developed multi-echo version of a center-out echo planar-imaging (EPI) readout was employed with pseudo-continuous arterial spin labeling (pCASL). It achieved very short echo and inter-echo times and facilitated a simultaneous detection of functional CBF and BOLD changes at 3 T with improved sensitivity. Evaluations of the absolute and relative changes of CBF and the effective transverse relaxation rate, [Formula], the coupling ratios, and their dependence on CBF at rest, CBFrest, indicated differences between activated and deactivated regions. Analysis of the shape of the respective functional responses also revealed faster negative responses with more pronounced post-stimulus transients. Resulting differences in the flow-metabolism coupling ratios were further examined for potential distinctions in the underlying neuronal contributions. HighlightsO_LIIntroduction of multi-echo center-out EPI for investigating concomitant CBF and BOLD changes in regions of positive (PBR) and negative BOLD response (NBR). C_LIO_LI{Delta}CBF timecourses closely follow those of [Formula] with negative signals exhibiting faster responses and more pronounced post-stimulus transients. C_LIO_LIDecreases in CBF appear to warrant a larger change in NBR than CBF increases in PBR regions. C_LIO_LIConsideration of baseline CBF values is important in comparisons of relative coupling ratios ({delta}sBOLD/{delta}cbf) between brain regions. C_LIO_LIDiscussion of potential excitatory and inhibitory neuronal feed forward control of CBF and CMRO2 in PBR and NBR. C_LI