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bioRxiv · 10.1101/2022.09.01.506297

Comparison of Culture Results between Specimens from Corneal Scraping with Microhomogenization and Corneal Swab in Moderate and Severe Bacterial Corneal Ulcers

Martha, F.·Edwar, L.·Karuniawati, A.·Fuady, A.·Tuasikal, R. M.
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Abstract

PurposeTo compare bacterial culture results from corneal infiltrate specimens taken by corneal scrapping followed by microhomogenization and direct scraping methods. MethodsThis study was a comparative cross-sectional, with 18 subjects divided into two randomized groups; Group A (first scrapings then swab), and group B (first swab then scrapings). Both scraping and swab methods are separated and then compared in data analysis. ResultsThe proportion of Gram according to culture in the scraping-microhomogenization technique was 6/13 (46.2%) and the proportion of Gram according to cultures in the swab technique was 5/13 (38.5%). McNemar test p-value is 1.000 (p>0.05). The proportion of positive cultures in the scraping-microhomogenization technique was 13/18 (72.2%) and the proportion of positive culture in swab technique was 9/18 (50%). McNemar test p-value is 0.219 (p> 0.05). The Kappa suitability test value from scraping-microhomogenization technique against the corneal swab is 0.333. ConclusionThe results of bacterial culture from specimens taken by the corneal scraping followed by microhomogenization have a higher positive culture compared to those taken by corneal swab, but not statistically significant.

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Martha, F., Edwar, L., Karuniawati, A., Fuady, A., Tuasikal, R. M.. 2022-09-02. Comparison of Culture Results between Specimens from Corneal Scraping with Microhomogenization and Corneal Swab in Moderate and Severe Bacterial Corneal Ulcers. https://doi.org/10.1101/2022.09.01.506297

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