Alpha kinase 3 signaling at the M-band maintains sarcomere integrity and proteostasis in striated muscle.

McNamara, J. W.; Parker, B. L.; Voges, H. K.; Mehdiabadi, N. R. R.; Bolk, F.; Chung, D. D.; Charitakis, N.; Molendijk, J.; Lal, S.; Ramialison, M.; Karavendzas, K.; Pointer, H. L.; Syrris, P.; Lopes, L. R.; Perry, E. M.; Lynch, G. S.; Mills, R. J.; Hudson, J. E.; Watt, K. I.; Porrello, E. R.; Elliott, D. A.

doi:10.1101/2022.09.01.506029

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bioRxiv · 10.1101/2022.09.01.506029

Alpha kinase 3 signaling at the M-band maintains sarcomere integrity and proteostasis in striated muscle.

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Abstract

Pathogenic variants in alpha kinase 3 (ALPK3) cause cardiomyopathy and musculoskeletal disease. How ALPK3 mutations result in disease remains unclear because little is known about this atypical kinase. Using a suite of engineered human pluripotent stem cells (hPSCs) we show that ALPK3 localizes to the M-Band of the sarcomere. ALPK3 deficiency disrupted sarcomeric organization and calcium kinetics in hPSC-derived cardiomyocytes and reduced force generation in cardiac organoids. Phosphoproteomic profiling identified ALPK3-dependant phospho-peptides that were enriched for sarcomeric components of the M-band and the ubiquitin-binding protein SQSTM1. Analysis of the ALPK3 interactome confirmed binding to M-band proteins including SQSTM1. Importantly, in hPSC-derived cardiomyocytes modeling ALPK3 deficiency and cardiomyopathic ALPK3 mutations, sarcomeric organization and M-band localization ofSQSTM1 were abnormal. These data suggest ALPK3 has an integral role in maintaining sarcomere integrity and proteostasis in striated muscle. We propose this mechanism may underly disease pathogenesis in patients with ALPK3 variants.

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McNamara, J. W., Parker, B. L., Voges, H. K., Mehdiabadi, N. R. R., Bolk, F., Chung, D. D., Charitakis, N., Molendijk, J., Lal, S., Ramialison, M., Karavendzas, K., Pointer, H. L., Syrris, P., Lopes, L. R., Perry, E. M., Lynch, G. S., Mills, R. J., Hudson, J. E., Watt, K. I., Porrello, E. R., Elliott, D. A.. 2022-09-03. Alpha kinase 3 signaling at the M-band maintains sarcomere integrity and proteostasis in striated muscle.. https://doi.org/10.1101/2022.09.01.506029

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