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bioRxiv · 10.1101/2022.08.28.505606

Graph neural networks ameliorate potential impacts of imprecise large-scale autonomous immunofluorescence labeling of immune cells on whole slide images

Abstract

The characteristics of tumor-infiltrating lymphocytes (TIL) are essential in cancer prognostication and treatment through the ability to indicate the tumors capacity to evade the immune system (e.g., as evidenced by nodal involvement). Machine learning technologies have demonstrated remarkable success for localizing TILs, though these methods require extensive curation of manual annotations or restaining procedures that can degrade tissue quality, resulting in imprecise annotation. In this study, we co-registered tissue slides stained for both hematoxylin and eosin (H&E) and immunofluorescence (IF) as means to rapidly perform large-scale annotation of nuclei. We integrated the following approaches to improve the prediction of TILs: 1) minimized tissue degradation on same-section tissue restaining, 2) developed a scoring algorithm to improve the selection of patches for machine learning modeling and 3) utilized a graph neural network deep learning approach to identify relevant contextual features for lymphocyte prediction. Our graph neural network approach accounts for surrounding contextual micro/macro-architecture tissue features to facilitate interpretation of registered IF. The graph neural network compares favorably (F1-score=0.9235, AUROC=0.9462) to two alternative modeling approaches. This study brings insight to the importance of contextual information leveraged from within and around neighboring cells in a nuclei classification workflow, as well as elucidate approaches which enable the rapid generation of large-scale annotations of lymphocytes for machine learning approaches for immune phenotyping. Such approaches can help further interrogate the spatial biology of colorectal cancer tumors and tumor metastasis.

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BibTeXRIS

Reddy, R., Sharma, C., Jackson, C., Palisoul, S., Barney, R., Kolling, F., Salas, L., Christensen, B., Brooks, G., Tsongalis, G., Vaickus, L., Levy, J.. 2022-08-28. Graph neural networks ameliorate potential impacts of imprecise large-scale autonomous immunofluorescence labeling of immune cells on whole slide images. https://doi.org/10.1101/2022.08.28.505606

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