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bioRxiv · 10.1101/2022.08.25.504658

Cross-amplified Barcodes on Slides for Spatial Transcriptomics Sequencing

JIN, Z.·Yu, N.·Bai, J.·Liu, Z.·Li, H.·Zhang, J.·Liang, C.
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Abstract

Spatial transcriptomics can reveal molecular signatures of tissue at spatial scales, but current technologies cannot integrate excellent accessibility and easy data-decoding. Here, we use oligonucleotides whose number is the square root of the number of spots to generate cross-amplified spatial barcodes on slides using microfluidic technology. This method can obtain microarrays with well-defined barcodes easily and at low cost, without post-decoding, which contributes to the popularization of spatial transcriptomics.

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JIN, Z., Yu, N., Bai, J., Liu, Z., Li, H., Zhang, J., Liang, C.. 2022-08-26. Cross-amplified Barcodes on Slides for Spatial Transcriptomics Sequencing. https://doi.org/10.1101/2022.08.25.504658

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