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bioRxiv · 10.1101/2022.08.24.505216

Synchrotron-based DEI and DEI-CT systems to image chick pea seeds, plant anatomy and the associated physiology at 30 keV.

Donepudi, V. R.
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Abstract

SummaryO_LIThe study the effect of contrast on seeds, growth and the associated anatomy and physiology, with upgraded imaging systems. The use of phase information to explore new information at various stage of the growth. C_LIO_LIThis work benefits, the use of Synchrotron-based DEI and DEI-CT systems to enhance the contrast in plant root architecture and contrast mechanisms, visibility of fine structures of root architecture growth and some aspects of physiology at acceptable level. These non-destructive, imaging systems available at the X-15A beamline, at NSLS, BNL, USA, are utilized. C_LIO_LINoticed detailed anatomical and physiological observations, contrast mechanisms, with these upgraded systems, compared to other conventional techniques, equipped with tube source of X-rays. Examined the potential of these systems to quantify the plant roots in situ. The acquired images provided good contrast, anatomical structures and physiology of the plant root micro-architecture. We observed some of the complex plant traits, such as growth, development, root architecture and the associated physiology. The interior structure, root architecture, root morphology, growth of laterals and subsequent laterals can be visualized directly by synchrotron-based imaging techniques. C_LIO_LIRoot architecture of the plant grown from seeds provides new information about the structure and enhancement of some desired property, for example, interior micro-structure of the root laterals and the subsequent laterals and the clear visibility of the leaves in detail. This way, it will be possible to differentiate the weakly and strongly attenuation of the signal traversing within the sample, clearly reflects the acceptable visibility in root laterals, subsequent laterals and the associated opaque matrix with enhanced contrast. The sample has a thin layer of hard structure outside and protein inside. Extinction properties of these samples will be characterized by Sy-DEI and Sy-DEI-CT. This way, we may be able to differentiate softly and weakly attenuation within the sample, to know more about the contrast mechanisms. The visibility, contrast and porosity, with finer details, can be noticed, with Sy-DEI-CT systems as distinguished from Sy-DEI. However, limited field of view, may limit the problems associated with Sy-DEI-CT. C_LI

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Donepudi, V. R.. 2022-08-26. Synchrotron-based DEI and DEI-CT systems to image chick pea seeds, plant anatomy and the associated physiology at 30 keV.. https://doi.org/10.1101/2022.08.24.505216

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