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bioRxiv · 10.1101/2022.08.19.504582

Physiological and functional heterogeneity in the mouse locus coeruleus

Tortorelli, L.·Mcburney-Lin, J.·Garad, M.·Megemont, M.·Riccomagno, M. M.·Yang, H.
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Abstract

Neurons in the locus coeruleus (LC) have been traditionally viewed as a homogenous population. Recent studies begin to reveal their heterogeneity at multiple levels, ranging from molecular compositions to projection targets. To further uncover variations of neuronal properties in the LC, we took a genetic-based tagging approach to identify these neurons. Our data revealed diverse spike waveforms among neurons in the LC region, including a considerable fraction of narrow-spiking units. While all wide-spiking units possessed the regular waveform polarity (negative-positive deflection), the narrow units can be further divided based on opposing waveform polarities. Under anesthesia, wide units emitted action potential at a higher rate than the narrow units. Under wakefulness, only one subtype of narrow units exhibited fast-spiking phenotype. These neurons also had long latencies to optogenetic stimulation. In-situ hybridization further supported the existence of a small population of putative GABAergic neurons in the LC core. Together, our data reveal characteristic differences among neurons in the LC region, and suggest that a fraction of electrophysiologically-identified narrow-spiking neurons can be fast-spiking interneurons, and their fast-spiking feature is masked by anesthesia.

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Tortorelli, L., Mcburney-Lin, J., Garad, M., Megemont, M., Riccomagno, M. M., Yang, H.. 2022-08-22. Physiological and functional heterogeneity in the mouse locus coeruleus. https://doi.org/10.1101/2022.08.19.504582

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