In airway epithelium, basal stem cells and their stress fibers remodel during the unjamming transition

Abstract

Under homeostatic conditions, epithelial cells remain non-migratory. However, during embryonic developmental and pathological processes, they become migratory. The mechanism underlying the transition between non-migratory and migratory epithelial cells is a fundamental question of cellular biology. In well-differentiated primary human bronchial epithelial cell layers, non-migratory epithelial cells become migratory through an unjamming transition (UJT). We have previously identified the hallmarks of UJT: apical cell elongation and collective cellular migration. These indicate that UJT is driven by intercellular force modulation, but the nature of these forces in pseudostratified epithelia is unknown. Here, we identify structural characteristics of basal stem cells that are indicative of force generation. During the UJT, basal stem cells elongate and enlarge, and their stress fibers lengthen and align. These morphological changes in basal stem cells correspond to the previously defined hallmarks of the UJT. Moreover, basal cell elongation and stress fiber lengthening precedes apical cell elongation. Together, these structural changes in basal stem cells suggest that in pseudostratified airway epithelium, basal stem cells may be the origin of the traction forces through stress fiber modeling during the UJT. Summary StatementOur image analysis of pseudostratified airway epithelium reveals basal stem cells as the likely source of traction forces driving collective cellular migration during an unjamming transition.