The Solanum americanum pangenome and effectoromics reveal new resistance genes against potato late blight

Lin, X.; Jia, Y.; Heal, R.; Prokchorchik, M.; Sindalovskaya, M.; Olave-Achury, A.; Makechemu, M.; Fairhead, S.; Noureen, A.; Heo, J.; Witek, K.; Smoker, M.; Taylor, J.; Shrestha, R.-K.; Lee, Y. Y.; Zhang, C.; Park, S. J.; sohn, k. h.; Huang, S.; Jones, J. D.

doi:10.1101/2022.08.11.503608

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bioRxiv · 10.1101/2022.08.11.503608

The Solanum americanum pangenome and effectoromics reveal new resistance genes against potato late blight

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Abstract

Late blight caused by the oomycete pathogen Phytophthora infestans continues to cause major worldwide losses in potato and tomato. Most accessions of Solanum americanum, a globally distributed, wild Solanaceae plant, are highly resistant to late blight. We generated high-quality reference genomes of four S. americanum accessions, re-sequenced 52 accessions, and we defined variation in the NLR immune receptor genes (the S. americanum NLRome). We further screened for variation in recognition of [~]315 P. infestans RXLR effectors in 52 S. americanum accessions. Using these genotypic and phenotypic data, we cloned three novel NLR-encoding genes Rpi-amr4, Rpi-amr16 and Rpi-amr17, and determined their corresponding RXLR effector genes Avramr4 (PITG_22825), Avramr16 (PITG_02860) and Avramr17 (PITG_04373) from P. infestans. These genomic resources and methodology will support efforts to convert potato into a "nonhost" of late blight and can be applied to diseases of other crops.

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Lin, X., Jia, Y., Heal, R., Prokchorchik, M., Sindalovskaya, M., Olave-Achury, A., Makechemu, M., Fairhead, S., Noureen, A., Heo, J., Witek, K., Smoker, M., Taylor, J., Shrestha, R.-K., Lee, Y. Y., Zhang, C., Park, S. J., sohn, k. h., Huang, S., Jones, J. D.. 2022-08-11. The Solanum americanum pangenome and effectoromics reveal new resistance genes against potato late blight. https://doi.org/10.1101/2022.08.11.503608

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