Acetylsalicylic acid and Salicylic acid inhibit SARS-CoV-2 replication in precision-cut lung slices

Geiger, N.; König, E.-M.; Oberwinkler, H.; Roll, V.; Diesendorf, V.; Faehr, S.; Obernolte, H.; Sewald, K.; Wronski, S.; Steinke, M.; Bodem, J.

doi:10.1101/2022.08.09.503270

bioRxiv · 10.1101/2022.08.09.503270

Acetylsalicylic acid and Salicylic acid inhibit SARS-CoV-2 replication in precision-cut lung slices

Geiger, N.·König, E.-M.·Oberwinkler, H.·Roll, V.·Diesendorf, V.·Faehr, S.·Obernolte, H.·Sewald, K.·Wronski, S.·Steinke, M.·Bodem, J.

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Abstract

Aspirin, with its active compound acetylsalicylic acid (ASA), shows antiviral activity against rhino- and influenza viruses and at high concentrations. We sought to investigate whether ASA and its metabolite salicylic acid (SA) inhibit SARS-CoV-2 since it might use similar pathways to influenza viruses. The compound-treated cells were infected with SARS-CoV-2. Viral replication was analyzed by RTqPCR. The compounds suppressed SARS-CoV-2 replication in cell culture cells and in a patient-near replication system using human precision-cut lung slices by two orders of magnitude. The compounds did not interfere with viral entry but led to lower viral RNA expression after 24 h.

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Geiger, N., König, E.-M., Oberwinkler, H., Roll, V., Diesendorf, V., Faehr, S., Obernolte, H., Sewald, K., Wronski, S., Steinke, M., Bodem, J.. 2022-08-09. Acetylsalicylic acid and Salicylic acid inhibit SARS-CoV-2 replication in precision-cut lung slices. https://doi.org/10.1101/2022.08.09.503270

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