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bioRxiv · 10.1101/2022.08.03.501714

Intermediate Filaments Associate with Aggresome-like Structures and Influence Rate of Cellular Expulsion of Neuronal Disease Aggregates

Abstract

Under conditions of proteostasis disequilibrium, neurons can enhance intracellular and extracellular protective mechanisms to guard against neurotoxicity. In mammals, an intracellular response to severe proteostasis imbalance that results from proteosome inhibition is the formation of juxtanuclear intermediate filament-surrounded, aggregate-filled aggresomes, which sequester threatening aggregates for later disposal via lysosomal degradation. Highly proteo-stressed neurons can also engage the assistance of neighboring cells in aggregate removal by loading threatening materials into large exopher vesicles that are transferred to neighboring cells for remote degradation of contents, a process that has been suggested to be analogous to the process that enables aggregate spreading in the human brain in neurodegenerative disease. In C. elegans these large extruded vesicles are called exophers. Here we document that players involved in aggresome biology are required for the elimination of potentially deleterious materials in neuronal exophers. We show that in proteostressed C. elegans touch receptor neurons, intermediate filament proteins IFD-1 and IFD-2 can assemble into juxtanuclear structures with multiple molecular and cellular characteristics of mammalian aggresomes. IFD-concentrating structures depend upon orthologs of mammalian adapter proteins, dynein motors, and microtubule integrity for aggregate collection into juxtanuclear compartments where they associate with ubiquitinated and neurotoxic polyglutamine expansion proteins. Strikingly, disruption of aggresome-decoration genes encoding IFDs or disruption of the BAG/14-3-3/Hsc70 adapter that promote aggregate loading of aggresome-like organelles, lowers exopher production via a cell autonomous mechanism. Although aggresome-like structures are not mandatory exopher cargo, IFD compartments can be extruded from neurons in exophers, revealing a previously unreported strategy to eliminate neuronal aggresome-like organelles via transfer to neighboring cells. Human IF neurofilament light chain hNFL can partially substitute for C. elegans IFD-2 proteins in promoting exopher production, indicating conservation of the capacity of intermediate filaments to influence neuronal aggregate extrusions across phyla. In sum, we identify a requirement for specific intermediate filaments, counterparts of human biomarkers of neuronal injury and disease and major components of Parkinsons disease Lewy bodies, in C. elegans neuronal aggresome-like organelle formation and large vesicle exopher extrusion from stressed neurons.

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BibTeXRIS

Arnold, M. L., Cooper, J. F., Androwski, R., Ardeshna, S., Melentijevic, I., Smart, A. J., Guasp, R. J., Nguyen, K. C., Bai, G., Hall, D. H., Grant, B. D., Driscoll, M.. 2022-08-05. Intermediate Filaments Associate with Aggresome-like Structures and Influence Rate of Cellular Expulsion of Neuronal Disease Aggregates. https://doi.org/10.1101/2022.08.03.501714

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