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bioRxiv · 10.1101/2022.07.28.501831

An Sfi1-like centrin-interacting centriolar plaque protein affects nuclear microtubule homeostasis.

Abstract

Malaria-causing parasites achieve rapid proliferation in human blood through multiple rounds of asynchronous nuclear division followed by daughter cell formation. Nuclear divisions critically depend on the centriolar plaque, which organizes intranuclear spindle microtubules. The centriolar plaque consists of an extranuclear compartment, which is connected via a nuclear pore-like structure to a chromatin-free intranuclear compartment. Composition and function of this non-canonical centrosome remain largely elusive. Centrins, which reside in the extranuclear part, are among the very few centrosomal proteins conserved in Plasmodium falciparum. Here we identify a novel centrin-interacting centriolar plaque protein. Conditional knock down of this Sfi1-like protein (PfSlp) caused a growth delay in blood stages, which correlated with a reduced number of daughter cells. Surprisingly, intranuclear tubulin abundance was significantly increased, which raises the hypothesis that the centriolar plaque might be implicated in regulating tubulin levels. Disruption of microtubule homeostasis caused polymerization of excess microtubules and aberrant mitotic spindles. Time-lapse microscopy revealed that this prevented or delayed spindle extension. Our study thereby identifies a novel extranuclear centriolar plaque factor and establishes a functional link to the intranuclear compartment of this divergent eukaryotic centrosome.

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BibTeXRIS

Wenz, C., Simon, C. S., Romao, T. P., Stuermer, V., Machado, M., Klages, N., Klemmer, A., Voss, Y., Ganter, M., Brochet, M., Guizetti, J.. 2022-07-29. An Sfi1-like centrin-interacting centriolar plaque protein affects nuclear microtubule homeostasis.. https://doi.org/10.1101/2022.07.28.501831

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