Efficient precision genome editing of Chlamydomonas reinhardtii with CRISPR/Cas

Nievergelt, A. P.; Diener, D.; Bogdanova, A.; Pigino, G.

doi:10.1101/2022.07.24.501283

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bioRxiv · 10.1101/2022.07.24.501283

Efficient precision genome editing of Chlamydomonas reinhardtii with CRISPR/Cas

Nievergelt, A. P.·Diener, D.·Bogdanova, A.·Pigino, G.

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Abstract

CRISPR/Cas genome engineering in the unicellular green algal model Chlamydomonas reinhardtii has until now only been applied to targeted gene disruption, whereas scar-less knock-in transgenesis has generally been considered infeasible. We have developed highly efficient homology-directed knock-in mutagenesis in cell-walled strains of Chlamydomonas. Our method allows scarless integration of fusion tags and sequence modifications of near arbitrary proteins without need for a preceding mutant line.

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Nievergelt, A. P., Diener, D., Bogdanova, A., Pigino, G.. 2022-07-24. Efficient precision genome editing of Chlamydomonas reinhardtii with CRISPR/Cas. https://doi.org/10.1101/2022.07.24.501283

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