Expansion of the Catalytic Repertoire of Alcohol Dehydrogenases in Plant Metabolism

Langley, C.; Tatsis, E. E.; Hong, B.; Nakamura, Y.; Paetz, C.; Stevenson, C. E. M.; Basquin, J.; Lawson, D. M.; Caputi, L.; O'Connor, S. E.

doi:10.1101/2022.07.24.501124

bioRxiv · 10.1101/2022.07.24.501124

Expansion of the Catalytic Repertoire of Alcohol Dehydrogenases in Plant Metabolism

Langley, C.·Tatsis, E. E.·Hong, B.·Nakamura, Y.·Paetz, C.·Stevenson, C. E. M.·Basquin, J.·Lawson, D. M.·Caputi, L.·O'Connor, S. E.

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Abstract

Medium-chain alcohol dehydrogenases (ADHs) comprise a highly conserved enzyme family that catalyse the reversible reduction of aldehydes. However, recent discoveries in plant natural product biosynthesis suggest that the catalytic repertoire of ADHs has been expanded. Here we report the crystal structure of dihydroprecondylocarpine acetate synthase (DPAS), an ADH that catalyses the non-canonical 1,4 reduction of an ,{beta}-unsaturated iminium moiety. Comparison with structures of plant-derived ADHs that catalyse 1,2-aldehyde and 1,2-iminium reductions suggest how the canonical ADH active site can be modified to carry out atypical carbonyl reductions, providing insight into how chemical reactions are diversified in plant metabolism.

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Langley, C., Tatsis, E. E., Hong, B., Nakamura, Y., Paetz, C., Stevenson, C. E. M., Basquin, J., Lawson, D. M., Caputi, L., O'Connor, S. E.. 2022-07-24. Expansion of the Catalytic Repertoire of Alcohol Dehydrogenases in Plant Metabolism. https://doi.org/10.1101/2022.07.24.501124

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