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bioRxiv · 10.1101/2022.07.19.500721

Site-specific Halogenation of Peptides and Proteins using engineered Halogenase Enzymes

Sana, B.·Ke, D.·Li, E. H. Y.·Ho, T.·Seayad, J.·Duong, H. A.·Ghadessy, F.
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Abstract

We demonstrate novel in vitro halogenation of peptides by halogenase enzymes, and identify the (G/S)GW motif (HaloTryp Tag) as a preferred substrate. We further derive PyrH halogenase mutants showing improved halogenation of the HaloTryp Tag, both as a free peptide and when genetically fused to model proteins.

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Sana, B., Ke, D., Li, E. H. Y., Ho, T., Seayad, J., Duong, H. A., Ghadessy, F.. 2022-07-20. Site-specific Halogenation of Peptides and Proteins using engineered Halogenase Enzymes. https://doi.org/10.1101/2022.07.19.500721

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