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bioRxiv · 10.1101/2022.07.17.500354

Identification of Long noncoding RNAs as substrate of cytoplasmic Capping Enzyme

Mukherjee, A.·Islam, S.·Mukherjee, C.
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Abstract

Cytoplasmic capping returns a cap to specific mRNAs thus protecting uncapped RNAs from decay. Prior to the identification of cytoplasmic capping, uncapped mRNAs were thought to be degraded. Here, we test whether long non-coding RNAs (lncRNAs) are cytoplasmic capping enzyme (cCE) substrates. The subcellular localization of fourteen lncRNAs associated with sarcomas were examined in U2OS osteosarcoma cells. We used 5 rapid amplification of cDNA ends (RACE) to assay uncapped forms of these lncRNAs. Inhibiting cytoplasmic capping elevated uncapped forms of selected lncRNAs indicating a plausible role of cCE in targeting them. Analysis of published cap analysis of gene expression (CAGE) data shows increased prevalence of certain 5-RACE cloned sequences suggesting that these uncapped lncRNAs are cytoplasmic capping targets.

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Mukherjee, A., Islam, S., Mukherjee, C.. 2022-07-17. Identification of Long noncoding RNAs as substrate of cytoplasmic Capping Enzyme. https://doi.org/10.1101/2022.07.17.500354

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