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bioRxiv · 10.1101/2022.07.15.500249

An oblique plane microscope for mesoscopic imaging of freely moving organisms with cellular resolution

Singh, R.·Subramanian, K.·Power, R. M.·Paix, A.·Ikmi, A.·Prevedel, R.
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Abstract

Several important questions in biology require non-invasive and three-dimensional imaging techniques with appropriate spatiotemporal resolution that permit live organisms to move in an unconstrained fashion over an extended field-of-view. While selective-plane illumination microscopy (SPIM) has emerged as a powerful method to observe live biological specimens at high spatio-temporal resolution, typical implementations often necessitate constraining sample mounting or lack the required volumetric speed. Here, we report on an open-top, dual-objective oblique plane microscope (OPM) capable of observing millimeter sized, freely moving animals at cellular resolution. We demonstrate the capabilities of our mesoscopic OPM (MesOPM) by imaging the behavioural dynamics of the sea anemone Nematostella vectensis over 1.56 x 1.56 x 0.25 mm at 1.5 x 2.8 x 5.3{micro}m resolution and 0.5Hz volume rate.

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Singh, R., Subramanian, K., Power, R. M., Paix, A., Ikmi, A., Prevedel, R.. 2022-07-16. An oblique plane microscope for mesoscopic imaging of freely moving organisms with cellular resolution. https://doi.org/10.1101/2022.07.15.500249

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