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bioRxiv · 10.1101/2022.07.14.500145

A case of mycobacteriosis associated with Mycobacterium pseudoshottsii in aquarium-reared fish in Japan

Komine, T.·Hyogo, I.·Kentaro, O.·Yoshida, M.·Sugimoto, Y.·Inohana, M.·Fukano, H.·Kurata, O.·Wada, S.
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Abstract

In 2019, several aquarium-reared fish died at a sea life park in Japan. Necropsy revealed micronodules on the spleen in the dotted gizzard shad (Konosirus punctatus). Seven of 16 fish exhibited microscopic multifocal granulomas associated with acid-fast bacilli in the spleen, kidney, liver, alimentary tract, mesentery, gills, and/or heart. Bacterial cultures yielded isolates from the dotted gizzard shad and a Japanese sardine (Sardinops melanostictus). Microbiological examination, multilocus sequence typing analysis, and variable number of tandem repeats analysis with a newly proposed 6-loci set revealed the isolates as Mycobacterium pseudoshottsii. To our knowledge, this is the first isolation of M. pseudoshottsii from aquarium-reared fish.

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Komine, T., Hyogo, I., Kentaro, O., Yoshida, M., Sugimoto, Y., Inohana, M., Fukano, H., Kurata, O., Wada, S.. 2022-07-15. A case of mycobacteriosis associated with Mycobacterium pseudoshottsii in aquarium-reared fish in Japan. https://doi.org/10.1101/2022.07.14.500145

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