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bioRxiv · 10.1101/2022.07.14.499815

Transcriptomic and proteomic profiling of NaV1.8-expressing mouse nociceptors

Schmidt, M.·Sondermann, J. R.·Gomez-Varela, D.·Millet, Q.·Wood, J. N.·Zhao, J.
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Abstract

Nociceptors play an essential role in both acute pain and chronic pain conditions. and have recently been classified into distinct subsets using single-cell transcriptional profiling. In this study, we examined protein levels in dorsal root ganglia using DIA Mass-spectrometry technologies with NaV1.8Cre+/-; ROSA26-flox-stop-flox-DTA (Diphtheria toxin fragment A) mutant mice (NaV1.8Cre-DTA), in which NaV1.8-expressing neurons (mainly nociceptors) in dorsal root ganglia (DRG) were ablated. The results show that 353 transcripts and 78 proteins, including nociceptor-specific sodium channels NaV1.8 (Scn10a) and NaV1.9 (Scn11a), were specifically expressed in nociceptors of DRG. A comparative analysis revealed that about 40% of nociceptor-specific proteins are shared within the nociceptor-specific transcript dataset. Scatter plots show that the proteome and transcriptome datasets in nociceptors have a moderate correlation (r = 0.4825), indicating the existence of post-transcriptional and post-translational gene regulation in nociceptors. This combined profiling study provides a unique resource for sensory studies, especially for pain research.

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Schmidt, M., Sondermann, J. R., Gomez-Varela, D., Millet, Q., Wood, J. N., Zhao, J.. 2022-07-16. Transcriptomic and proteomic profiling of NaV1.8-expressing mouse nociceptors. https://doi.org/10.1101/2022.07.14.499815

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