Identification of leukemia-associated immunophenotypes by database-guided flow cytometry provides a highly sensitive and reproduciblestrategy for the study of measurable residual disease in acutemyeloblastic leukemia

Pinero, P.; Morillas, M.; Gutierrez, N.; Barragan, E.; Such, E.; Brena, J.; Gil, C.; Garcia, C.; Botella, C.; Gonzalez-Navajas, J. M.; Zapater, P.; Montesinos, P.; Sempere, A.; Tarin, F.

doi:10.1101/2022.07.12.499672

bioRxiv · 10.1101/2022.07.12.499672

Identification of leukemia-associated immunophenotypes by database-guided flow cytometry provides a highly sensitive and reproduciblestrategy for the study of measurable residual disease in acutemyeloblastic leukemia

Pinero, P.·Morillas, M.·Gutierrez, N.·Barragan, E.·Such, E.·Brena, J.·Gil, C.·Garcia, C.·Botella, C.·Gonzalez-Navajas, J. M.·Zapater, P.·Montesinos, P.·Sempere, A.·Tarin, F.

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Abstract

BackgroundMultiparametric Flow Cytometry (MFC) is an essential tool to study the involved cell lineages, the aberrant differentiation/maturation patterns and the expression of aberrant antigens in acute myeloid leukemia (AML). The characterization of leukemia-associated immunophenotypes (LAIPs) at the moment of diagnosis is critical to establish reproducible strategies for the study of measurable residual disease using MFC (MFC-MRD). MethodsIn this study, we identified and characterized LAIPs by comparing the leukemic populations of 145 AML patients, using the EuroFlow AML/ MDS MFC panel, with 6 databases of normal myeloid progenitors (MPCs). Principal component analysis was used to identify and characterize the LAIPs, which were then used to generate individual profiles for MFC-MRD monitoring. Furthermore, we investigated the relationship between the expression patterns of LAIPs and the different subtypes of AML. The MFC-MRD study was performed by identifying residual AML populations that matched with the LAIPs at diagnosis. To further validate this approach, the presence of MRD was also assessed by qPCR (qPCR-MRD). Finally, we studied the association between MFC-MRD and progression-free survival (PFS). ResultsThe strategy used in this study allowed us to describe more than 300 different LAIPs and facilitated the association of specific phenotypes with certain subtypes of AML. The MFC-MRD monitoring based on LAIPs with good/strong specificity was applicable to virtually all patients and showed a good correlation with qPCR-MRD and PFS. ConclusionsThe described methodology provides an objective method to identify and characterize LAIPs. Furthermore, it provides a theoretical basis to develop highly sensitive MFC-MRD strategies.

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Pinero, P., Morillas, M., Gutierrez, N., Barragan, E., Such, E., Brena, J., Gil, C., Garcia, C., Botella, C., Gonzalez-Navajas, J. M., Zapater, P., Montesinos, P., Sempere, A., Tarin, F.. 2022-07-13. Identification of leukemia-associated immunophenotypes by database-guided flow cytometry provides a highly sensitive and reproduciblestrategy for the study of measurable residual disease in acutemyeloblastic leukemia. https://doi.org/10.1101/2022.07.12.499672

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