Proteomic profile of nuclei containing p62-positive inclusions in neuronal intranuclear inclusion disease

Kurihara, M.; Mano, T.; Eto, F.; Yao, I.; Sato, K.; Ohtomo, G.; Bannai, T.; Shibata, S.; Ishiura, H.; Ikemura, M.; Matsubara, T.; Morishima, M.; Saito, Y.; Murayama, S.; Toda, T.; Setou, M.; Iwata, A.

doi:10.1101/2022.07.08.499227

bioRxiv · 10.1101/2022.07.08.499227

Proteomic profile of nuclei containing p62-positive inclusions in neuronal intranuclear inclusion disease

Kurihara, M.·Mano, T.·Eto, F.·Yao, I.·Sato, K.·Ohtomo, G.·Bannai, T.·Shibata, S.·Ishiura, H.·Ikemura, M.·Matsubara, T.·Morishima, M.·Saito, Y.·Murayama, S.·Toda, T.·Setou, M.·Iwata, A.

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Abstract

Neuronal intranuclear inclusion disease (NIID) is a neurodegenerative disease characterized by eosinophilic hyaline intranuclear inclusions in the neurons, glial cells, and other somatic cells. Although CGG repeat expansions in NOTCH2NLC have been identified in most East Asian patients with NIID, the pathophysiology of NIID remains unclear. Intranuclear inclusions are the pathological hallmark of NIID. Previously, immunohistochemistry has revealed that intranuclear inclusions are ubiquitin-, p62-, and SUMO-1-positive, suggesting the possible alteration of the ubiquitin-proteasomal protein degradation system in the nuclei with inclusions. However, the molecular mechanisms within these nuclei remain unclear. Herein, we analyzed the proteomic profile of nuclei with p62-positive inclusions in NIID with CGG repeat expansion in NOTCH2NLC to discover the proteins involved in the NIID pathophysiology. We used fluorescence-activated cell sorting and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify each protein identified in the nuclei with p62-positive inclusions. The distribution of increased proteins in the autopsy brain samples from three NIID patients was confirmed via immunofluorescence. Overall, 526 proteins were identified, of which 243 were consistently quantified using MS. A 1.4-fold increase was consistently observed for 20 proteins in nuclei with p62-positive inclusions compared to those without. Fifteen proteins identified with medium or high confidence in the LC-MS/MS analysis were further evaluated. Gene ontology enrichment analysis showed enrichment of several terms, including poly(A) RNA binding, nucleosomal DNA binding, and protein binding. Immunofluorescence studies confirmed that the fluorescent intensities of increased RNA-binding proteins identified by proteomic analysis, namely hnRNP A3, hnRNP A2/B1, and hnRNP C1/C2, were higher in the nuclei with p62-positive inclusions than those without; however, they were not confined to the intranuclear inclusions. We identified several increased proteins in nuclei with p62-positive inclusions. These data can form the basis for understanding the pathophysiology of NIID.

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Kurihara, M., Mano, T., Eto, F., Yao, I., Sato, K., Ohtomo, G., Bannai, T., Shibata, S., Ishiura, H., Ikemura, M., Matsubara, T., Morishima, M., Saito, Y., Murayama, S., Toda, T., Setou, M., Iwata, A.. 2022-07-09. Proteomic profile of nuclei containing p62-positive inclusions in neuronal intranuclear inclusion disease. https://doi.org/10.1101/2022.07.08.499227

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