Architecture and dynamics of a novel desmosome-endoplasmic reticulum organelle

Bharathan, N. K.; Giang, W.; Aaron, J. S.; Khuon, S.; Chew, T.-L.; Preibisch, S.; Trautman, E. T.; Heinrich, L.; Bogovic, J.; Bennett, D.; Ackerman, D.; Park, W.; Petruncio, A.; Weigel, A. V.; Saalfeld, S.; COSEM Project Team,; Vogl, A. W.; Stahley, S. N.; Kowalczyk, A. P.

doi:10.1101/2022.07.07.499185

bioRxiv · 10.1101/2022.07.07.499185

Architecture and dynamics of a novel desmosome-endoplasmic reticulum organelle

Bharathan, N. K.·Giang, W.·Aaron, J. S.·Khuon, S.·Chew, T.-L.·Preibisch, S.·Trautman, E. T.·Heinrich, L.·Bogovic, J.·Bennett, D.·Ackerman, D.·Park, W.·Petruncio, A.·Weigel, A. V.·Saalfeld, S.·COSEM Project Team,·Vogl, A. W.·Stahley, S. N.·Kowalczyk, A. P.

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Abstract

The endoplasmic reticulum (ER) forms a dynamic network that contacts other cellular membranes to regulate stress responses, calcium signaling, and lipid transfer. Using high-resolution volume electron microscopy, we find that the ER forms a previously unknown association with keratin intermediate filaments and desmosomal cell-cell junctions. Peripheral ER assembles into mirror image-like arrangements at desmosomes and exhibits nanometer proximity to keratin filaments and the desmosome cytoplasmic plaque. ER tubules exhibit stable associations with desmosomes, and perturbation of desmosomes or keratin filaments alters ER organization and mobility. These findings indicate that desmosomes and the keratin cytoskeleton pattern the distribution of the ER network. Overall, this study reveals a previously unknown subcellular architecture defined by the structural integration of ER tubules with an epithelial intercellular junction. One-Sentence SummaryThe desmosome adhesive junction regulates the organization and dynamics of the endoplasmic reticulum network.

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Bharathan, N. K., Giang, W., Aaron, J. S., Khuon, S., Chew, T.-L., Preibisch, S., Trautman, E. T., Heinrich, L., Bogovic, J., Bennett, D., Ackerman, D., Park, W., Petruncio, A., Weigel, A. V., Saalfeld, S., COSEM Project Team,, Vogl, A. W., Stahley, S. N., Kowalczyk, A. P.. 2022-07-08. Architecture and dynamics of a novel desmosome-endoplasmic reticulum organelle. https://doi.org/10.1101/2022.07.07.499185

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