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bioRxiv · 10.1101/2022.07.05.498330

Direct Cell Extraction of Membrane Proteins for Structure-Function Analysis

Drulyte, I.·Gutgsell, A. R.·Lloris-Garcer, P.·Liss, M.·Geschwindner, S.·Radjainia, M.·Frauenfeld, J.·Loving, R.
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Abstract

Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structurefunction analysis using SPR and cryo-EM.

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Drulyte, I., Gutgsell, A. R., Lloris-Garcer, P., Liss, M., Geschwindner, S., Radjainia, M., Frauenfeld, J., Loving, R.. 2022-07-05. Direct Cell Extraction of Membrane Proteins for Structure-Function Analysis. https://doi.org/10.1101/2022.07.05.498330

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