Vast human gut virus diversity uncovered by combined short- and long-read sequencing

Chen, W.; Chen, J.; Sun, C.; Dong, Y.; Jin, M.; Lai, S.; Jia, L.; Zhao, X.; Gao, N. L.; Liu, Z.; Bork, P.; Zhao, X.-M.

doi:10.1101/2022.07.03.498593

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bioRxiv · 10.1101/2022.07.03.498593

Vast human gut virus diversity uncovered by combined short- and long-read sequencing

Chen, W.·Chen, J.·Sun, C.·Dong, Y.·Jin, M.·Lai, S.·Jia, L.·Zhao, X.·Gao, N. L.·Liu, Z.·Bork, P.·Zhao, X.-M.

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Abstract

Current metagenome-assembled human phage catalogs contained mostly fragmented genomes. Here, we developed a vigorous phage detection method involving phage enrichment and long-read sequencing and applied to 135 fecal samples. With ~10 times more efficient in obtaining complete genomes (~34%) than the Gut Virome Database, we identified the first megabasephage (~1.03Mb), and revealed the hidden diversity of the gut phageome including dozens of phages more prevalent than the crAssphages and Gubaphages.

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Chen, W., Chen, J., Sun, C., Dong, Y., Jin, M., Lai, S., Jia, L., Zhao, X., Gao, N. L., Liu, Z., Bork, P., Zhao, X.-M.. 2022-07-04. Vast human gut virus diversity uncovered by combined short- and long-read sequencing. https://doi.org/10.1101/2022.07.03.498593

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