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bioRxiv · 10.1101/2022.06.23.497420

OligoFlow: rapid and sensitive virus quantification using flow cytometry and oligonucleotide hybridization

Paterson, J. S.·Dann, L. M.·Carlson-Jones, J.·Giles, S. K.·McIvor, C.·Speck, P. G.·Mitchell, J. G.
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Abstract

Flow cytometry is an established method for the detection and enumeration of viruses. However, the technique is unable to target specific viral species. Here, we present OligoFlow, a novel method for the rapid detection and enumeration of viruses by incorporating flow cytometry with species specific oligonucleotide hybridization. Using Ostried herpesvirus and dengue virus as model organisms, we demonstrate high-level detection and specificity. Our results represent a significant advancement in viral flow cytometry, opening the possibilities for the rapid identification of viruses in time critical settings.

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Paterson, J. S., Dann, L. M., Carlson-Jones, J., Giles, S. K., McIvor, C., Speck, P. G., Mitchell, J. G.. 2022-06-26. OligoFlow: rapid and sensitive virus quantification using flow cytometry and oligonucleotide hybridization. https://doi.org/10.1101/2022.06.23.497420

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