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bioRxiv · 10.1101/2022.06.22.497178

Identification of Circular Dorsal Ruffles as Signal Platforms for the AKT pathways in Glomerular Podocytes

Abstract

Circular dorsal ruffles (CDRs) are rounded membrane ruffles induced by growth factors to function as precursors of the large-scale endocytosis called macropinocytosis. In cell line systems, CDR/macropinocytosis regulate the AKT-mTORC1 pathway, a canonical growth factor signaling. However, it is not known if this mechanism occurs in tissues. Here, utilizing ultra-high-resolution scanning electron microscopy, we report that CDRs are expressed in glomerular podocytes ex vivo and in vivo. Biochemical and imaging analysis revealed that AKT phosphorylation is localized to CDRs upstream of mTORC1 activation in podocyte cell line and isolated glomeruli, indicating that CDRs function as signal platforms for AKT-mTORC1 pathway in podocytes to maintain the kidney function. Because mTORC1 has critical roles in the podocyte metabolism and the aberrant activation of mTORC1 triggers podocytopathies, these results suggest that targeting CDR formation would be a potential therapeutic approach for the diseases.

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Yoshida, S., Hua, R., Torres, M., Sun, X., Wei, J., Wang, L., Inoki, K.. 2022-06-23. Identification of Circular Dorsal Ruffles as Signal Platforms for the AKT pathways in Glomerular Podocytes. https://doi.org/10.1101/2022.06.22.497178

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