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bioRxiv · 10.1101/2022.06.22.497080

Single molecule methylation profiles of cell-free DNA in cancer with nanopore sequencing

Lau, B. T.·Almeda, A.·Schauer, M.·McNamara, M.·Bai, X.·Meng, Q.·Partha, M.·Grimes, S. M.·Lee, H.·Heestand, G. M.·Ji, H. P.
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Abstract

Epigenetic characterization of cell-free DNA (cfDNA) is an emerging approach for characterizing diseases. We developed a strategy using nanopore-based single-molecule sequencing to measure cfDNA methylomes. This approach generated up to hundreds of millions of reads per cfDNA sample from cancer patients, an order of magnitude improvement over existing nanopore methods. Leveraging methylomes of matched tumors and immune cells, we characterized cfDNA methylomes of cancer patients for longitudinal monitoring during treatment.

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Lau, B. T., Almeda, A., Schauer, M., McNamara, M., Bai, X., Meng, Q., Partha, M., Grimes, S. M., Lee, H., Heestand, G. M., Ji, H. P.. 2022-06-26. Single molecule methylation profiles of cell-free DNA in cancer with nanopore sequencing. https://doi.org/10.1101/2022.06.22.497080

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