A G protein coupled receptor like protein systematically modulates nutrient-growth by Ca2+ mediated phosphatidylcholine perception during evolutionary adaptation

Huang, Z.; Tong, Y.; He, X.; Teng, W.; Hu, M.; Li, H.; Zhang, Y.; Li, J.; Zhao, X.

doi:10.1101/2022.06.17.496194

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bioRxiv · 10.1101/2022.06.17.496194

A G protein coupled receptor like protein systematically modulates nutrient-growth by Ca2+ mediated phosphatidylcholine perception during evolutionary adaptation

Huang, Z.·Tong, Y.·He, X.·Teng, W.·Hu, M.·Li, H.·Zhang, Y.·Li, J.·Zhao, X.

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Abstract

Nutrients are important for growth in both plants and animals, uncovering of signaling pathway in nutrients determined growth is essential. Here we cloned TaPCGR1-3B (Phospholipid Coordinated Growth and nutrients Response 1), controlled by SNPs on alternative splicing and transcription factors binding, conferring nitrogen deficiency response. TaPCGR1-3B was localized in plasma membrane and endoplasmic reticulum of meristem cell. Nitrogen deprivation stimulated interaction of TaPCGR1-3B with G protein alpha subunit and phospholipase C 9, which was inhibited by phosphatidylcholine, to trigger Ca2+ signaling and inhibit normal growth. Knockdown of TaPCGR1 rescued the growth inhibition caused by nutrient deficient conditions by modulation of phosphatidylcholine induced growth gene expression through Ca2+ signaling disruption. Modulating of phosphatidylcholine mediated TaPCGR1 activity thus tightly regulated growth through Ca2+ signaling.

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Huang, Z., Tong, Y., He, X., Teng, W., Hu, M., Li, H., Zhang, Y., Li, J., Zhao, X.. 2022-06-17. A G protein coupled receptor like protein systematically modulates nutrient-growth by Ca2+ mediated phosphatidylcholine perception during evolutionary adaptation. https://doi.org/10.1101/2022.06.17.496194

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