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bioRxiv · 10.1101/2022.06.16.496383

Development and Evaluation of RT-LAMP Assays to Identify Variants of SARS-CoV-2

Park, G.-S.·Kim, S.-J.·Maeng, J.-S.
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Abstract

Emergence of new variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) during current pandemic of Coronavirus Disease 2019 (COVID-19) and several waves of infections by some of variants emphasized the importance of continuous surveillance. While genomic surveillance through whole genome sequencing is performed as a standard method, identification of known variants through mutation-targeting molecular diagnosis such as qRT-PCR is also useful for timely investigation. However, there are limited studies regarding the concurrent detection and identification of SARS-CoV-2 variants through a LAMP-based method. In this study, we developed and evaluated RT-LAMP assays to detect characteristic deletions of SARS-CoV-2 variants. In addition, we evaluated a fluorescent probe mediated method for identification of single nucleotide substitution by RT-LAMP. Finally, we discussed restrictions and perspectives regarding pathogen screening and surveillance of variants by RT-LAMP based on our observations.

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Park, G.-S., Kim, S.-J., Maeng, J.-S.. 2022-06-16. Development and Evaluation of RT-LAMP Assays to Identify Variants of SARS-CoV-2. https://doi.org/10.1101/2022.06.16.496383

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