Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus

Kaplan, M.; Chang, Y.-W.; Oikonomou, C. M.; Nicolas, W. J.; Jewett, A. I.; Kreida, S.; Dutka, P.; Rettberg, L. A.; Maggi, S.; Jensen, G. J.

doi:10.1101/2022.06.13.496000

bioRxiv · 10.1101/2022.06.13.496000

Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus

Kaplan, M.·Chang, Y.-W.·Oikonomou, C. M.·Nicolas, W. J.·Jewett, A. I.·Kreida, S.·Dutka, P.·Rettberg, L. A.·Maggi, S.·Jensen, G. J.

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Abstract

Bdellovibrio bacteriovorus is an endobiotic microbial predator that offers promise as a living antibiotic for its ability to kill Gram-negative bacteria, including human pathogens. Even after six decades of study, fundamental details of its predation cycle remain mysterious. Here, we used cryo-electron tomography to comprehensively image the lifecycle of B. bacteriovorus at nanometer-scale resolution. In addition to providing the first high-resolution images of predation in a native (hydrated, unstained) state, we also discover several surprising features of the process, including novel macromolecular complexes involved in prey attachment/invasion and a flexible portal structure lining a hole in the prey peptidoglycan that tightly seals the prey outer membrane around the predator during entry. Unexpectedly, we find that B. bacteriovorus does not shed its flagellum during invasion, but rather resorbs it into its periplasm for degradation. Finally, following replication and division in the bdelloplast, we observe a transient and extensive ribosomal lattice on the condensed B. bacteriovorus nucleoid. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=200 SRC="FIGDIR/small/496000v1_ufig1.gif" ALT="Figure 1"> View larger version (46K): org.highwire.dtl.DTLVardef@35da74org.highwire.dtl.DTLVardef@19923f1org.highwire.dtl.DTLVardef@106a616org.highwire.dtl.DTLVardef@181f2fb_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Kaplan, M., Chang, Y.-W., Oikonomou, C. M., Nicolas, W. J., Jewett, A. I., Kreida, S., Dutka, P., Rettberg, L. A., Maggi, S., Jensen, G. J.. 2022-06-13. Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus. https://doi.org/10.1101/2022.06.13.496000

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