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bioRxiv · 10.1101/2022.06.13.495975

CaV1.2 channelopathic mutations evoke diverse pathophysiological mechanisms

Abstract

The first pathogenic mutation in CaV1.2 was identified in 2004 and was shown to cause a severe multisystem disorder known as Timothy syndrome (TS). The mutation was localized to the distal S6 region of the channel, a region known to play a major role in channel activation. TS patients suffer from life-threatening cardiac symptoms as well as significant neurodevelopmental deficits including autism spectrum disorder (ASD). Since this discovery, the number and variety of mutations identified in CaV1.2 has grown tremendously, and the distal S6 regions remains a frequent locus for many of these mutations. While the majority of patients harboring these mutations exhibit cardiac symptoms which can be well explained by known pathogenic mechanisms, the same cannot be said for the ASD or neurodevelopmental phenotypes seen in some patients, indicating a gap in our understanding of the pathogenesis of CaV1.2 channelopathies. Here, we use of whole cell patch clamp, quantitative Ca2+ imaging, and single channel recordings to expand the known mechanisms underlying the pathogenesis of CaV1.2 channelopathies. Specifically, we find that mutations within the S6 region can exert independent and separable effects on activation, voltage-dependent inactivation (VDI) and Ca2+-dependent inactivation (CDI). Moreover, the mechanisms underlying the CDI effects of these mutations are varied and include altered channel opening and possible disruption of CDI transduction. Overall, these results provide a structure-function framework to conceptualize the role of S6 mutations in pathophysiology and offer insight into the biophysical defects associated with distinct clinical manifestations.

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BibTeXRIS

Bamgboye, M. A., Herold, K. G., Vieira, D. C. O., Traficante, M. K., Rogers, P. J., Ben-Johny, M., Dick, I. E.. 2022-06-16. CaV1.2 channelopathic mutations evoke diverse pathophysiological mechanisms. https://doi.org/10.1101/2022.06.13.495975

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