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bioRxiv · 10.1101/2022.06.08.495306

p115RhoGEF activates RhoA to support tight junction maintenance and remodeling

Chumki, S. A.·van den Goor, L. M.·Hall, B. N.·Miller, A. L.
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Abstract

In vertebrates, epithelial cell-cell junctions must rapidly remodel to maintain barrier function as cells undergo dynamic shape-change events. Consequently, localized leaks sometimes arise within the tight junction (TJ) barrier, which are repaired by short-lived activations of RhoA, called "Rho flares". However, what activates RhoA at leak sites remains unknown. Here, we asked which guanine nucleotide exchange factor (GEF) localizes to TJs to initiate Rho activity at Rho flares. We find that p115RhoGEF locally activates Rho flares at sites of barrier leaks and TJ loss. Knockdown of p115RhoGEF leads to diminished Rho flare intensity and impaired TJ remodeling. p115RhoGEF knockdown also decreases junctional active RhoA levels, thus compromising the apical actomyosin array and junctional complex. Furthermore, p115RhoGEF is necessary to preserve global TJ barrier function by promoting local leak repair and preventing repeating leaks. In all, our work demonstrates a central role for p115RhoGEF in activating junctional RhoA to preserve barrier function and direct local TJ remodeling.

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Chumki, S. A., van den Goor, L. M., Hall, B. N., Miller, A. L.. 2022-06-08. p115RhoGEF activates RhoA to support tight junction maintenance and remodeling. https://doi.org/10.1101/2022.06.08.495306

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