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bioRxiv · 10.1101/2022.06.06.494947

Identification of ASPDH as a novel NAADP-binding protein

He, X.·Kang, Y.·Chen, L.
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Abstract

NAADP is a signaling molecule that can induce calcium release from intracellular acidic stores. However, proteins that bind to NAADP are understudied. Here, we identify ASPDH as an NAADP-binding protein through biochemical purification from pig livers. ITC experiment using the recombinantly expressed protein shows a 1:1 binding stoichiometry and a Kd of 455 nM between NAADP and mouse ASPDH. In contrast, recombinantly expressed JPT2 and LSM12, two proteins previously identified as NAADP-receptors, show no binding in ITC experiments.

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He, X., Kang, Y., Chen, L.. 2022-06-06. Identification of ASPDH as a novel NAADP-binding protein. https://doi.org/10.1101/2022.06.06.494947

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