Proteome-wide microarray-based screening of PAR-binding proteins

Kang, B. G.; Kang, S.-U.; Kim, J. J.; Kwon, J.-S.; Gagne, J.-P.; Lee, S. Y.; Kim, S.; Sangwon, K. L.; Ha, S.; Jeong, J. S.; Lee, Y.-I.; Zhu, H.; Kim, D.; Poirier, G. G.; Kang, H. C.; Dawson, V. L.; Dawson, T. M.

doi:10.1101/2022.06.06.494829

bioRxiv · 10.1101/2022.06.06.494829

Proteome-wide microarray-based screening of PAR-binding proteins

Kang, B. G.·Kang, S.-U.·Kim, J. J.·Kwon, J.-S.·Gagne, J.-P.·Lee, S. Y.·Kim, S.·Sangwon, K. L.·Ha, S.·Jeong, J. S.·Lee, Y.-I.·Zhu, H.·Kim, D.·Poirier, G. G.·Kang, H. C.·Dawson, V. L.·Dawson, T. M.

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Abstract

Poly(ADP-ribose) (PAR) plays a crucial role in intracellular signaling and scaffolding through covalent modification or non-covalent binding to target proteins. The non- covalent binding PARylome has not been extensively characterized. Here we performed a PAR-binding screen using a human protein microarray that covers most of the human proteome to characterize the non-covalent binding PARylome. A total of 356 PAR- binding proteins were identified. The PAR-binding PARylome suggests that PAR- binding regulates a variety of biological processes beyond well-characterized DNA damage signaling and DNA repair. Proteins that may be reprogrammed by PAR-binding include signaling molecules, transcription factors, nucleic acid binding proteins, calcium binding proteins, ligases, oxidoreductases, enzymes, transferases, hydrolases, and receptors. The global database of PAR-binding proteins that we established will be a valuable tool for further in-depth analysis of the role of PARylation in a wide range of biological contexts.

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Kang, B. G., Kang, S.-U., Kim, J. J., Kwon, J.-S., Gagne, J.-P., Lee, S. Y., Kim, S., Sangwon, K. L., Ha, S., Jeong, J. S., Lee, Y.-I., Zhu, H., Kim, D., Poirier, G. G., Kang, H. C., Dawson, V. L., Dawson, T. M.. 2022-06-06. Proteome-wide microarray-based screening of PAR-binding proteins. https://doi.org/10.1101/2022.06.06.494829

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